Journal: PLoS ONE

Article Title: Fluorescence2D: Software for Accelerated Acquisition and Analysis of Two-Dimensional Fluorescence Spectra

doi: 10.1371/journal.pone.0101227

Figure Lengend Snippet: The interactive tools of MATLAB allow for easy adjustment, inspection, and export of the graphics created by Fluorescence2D . In the panel A , arrows indicate locations of the zoom tool ( 1 ) used to enlarge the desired area, the data inspector tool ( 2 ) allowing to determine values of wavelength and intensity at any specific point, and the interactive graphics mode toggle ( 3 ) for adjustment of font sizes, labels, ticks, and colors. Export of the final image into a desired format is performed through File:Save As menu item as shown in Panel B .

Article Snippet: In this case, the user would edit the corresponding MATLAB figure using the MATLAB interactive graphics toolbar ( ).

Techniques:

Journal: PLoS ONE

Article Title: Fluorescence2D: Software for Accelerated Acquisition and Analysis of Two-Dimensional Fluorescence Spectra

doi: 10.1371/journal.pone.0101227

Figure Lengend Snippet: (Panel A ) The 2D spectrum of the buffer solution (without baseline subtraction) is shown contoured between 0 and 10% of total intensity (blue to red). The first order reflection, 1 ; the second order reflection, 2 ; Raman scattering, 3 . The black solid line separates the lower area with experimental data from the upper area added to make the dataset rectangular for visualization. (Panel B ) The 2D spectrum of H-Ras in the complex with mant-GDP. The spectrum of the buffer solution (from Panel A ) was subtracted as a baseline; the resulting graph was contoured from 3 to 13% of total intensity scale. Tyrosine fluorescence, 4 ; its second order reflection, 5 ; mant-GDP emission, 6 and 7 . Spectra in both panels were “zoomed in” using interactive MATLAB graphics tools. The original and processed experimental data along with the corresponding fluorescence2Dmain.m script are included with the Fluorescence2D package in the sample_data/ folder.

Article Snippet: In this case, the user would edit the corresponding MATLAB figure using the MATLAB interactive graphics toolbar ( ).

Techniques: Fluorescence

Journal: STAR Protocols

Article Title: Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics

doi: 10.1016/j.xpro.2022.101433

Figure Lengend Snippet:

Article Snippet: Statistical computing and graphics software (MATLAB) , This paper , mathworks.com.

Techniques: Recombinant, Sterility, Saline, Transferring, Hood, Light Microscopy, Fluorescence, Microscopy, Software